FB2021_02, released April 13, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
p53, mrn
FlyBase ID
FBig0000098430
Interaction Network
Interactions Browser links
p53 network
mrn network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221828-1.coIP.WB
Reference
Villicaña et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
p53 

Dp53

bait

V5 tag

mrn 

Dmp52

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Villicaña et al., 2013)
FBrf0221828-10.PD.A
Reference
Villicaña et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mrn 

Dmp52

bait

GST tag

mrn 
mrn 
p53 

Dp53

prey

[35]S label

p53 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mrn 
N-terminal region
sufficient binding region
aa 1-407
mrn 
C-terminal region
sufficient binding region
aa 408-499
p53 
DNA binding domain
sufficient binding region
aa 1-307

coordinates relative to shorter 385 residue isoform

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Villicaña et al., 2013)

mrn has at least two distinct p53 binding sites.

(Villicaña et al., 2013)
FBrf0221828-2.coIP.WB
Reference
Villicaña et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
mrn 

Dmp52

bait

FLAG tag

p53 

Dp53

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Villicaña et al., 2013)
FBrf0221828-5.coIP.WB
Reference
Villicaña et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
p53 

Dp53

bait

FLAG tag

mrn 

Dmp52

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Villicaña et al., 2013)
FBrf0221828-6.coIP.WB
Reference
Villicaña et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
p53 

Dp53

bait
mrn 

Dmp52

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Villicaña et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)