FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Egfr, kek1
FlyBase ID
FBig0000098456
Interaction Network
Interactions Browser links
Egfr network
kek1 network
Reported Interactions
FBrf0162074-1.coIP.WB
Reference
Ghiglione et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kek1 

Kek1

bait

MYC tag

kek1 
Egfr 

DER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kek1 
Leucine Rich Repeats
necessary binding region
aa 126-277

coordinates relative to kek1-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ghiglione et al., 2003)
FBrf0174701-1.coIP.WB
Reference
Alvarado et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S3
Participants
Corresponds to
Reported as
Role
Note
kek1 

Kek1

bait

GFP tag

kek1 
kek1 
kek1 
Egfr 

EGFR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kek1 
point mutation L136F
mutation decreasing interaction
aa 136

L136F

kek1 
point mutation G160S
mutation disrupting interaction
aa 160

G160S

kek1 
point mutation G160D
mutation disrupting interaction
aa 160

G160D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S3 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Alvarado et al., 2004)
FBrf0174702-1.coIP.WB
Reference
Derheimer et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S3
Participants
Corresponds to
Reported as
Role
Note
kek1 

Kek1

bait

GFP tag

Egfr 

EGFR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S3 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Derheimer et al., 2004)
FBrf0178972-1.coIP.WB
Reference
Alvarado et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S3
Participants
Corresponds to
Reported as
Role
Note
kek1 

Kek1

bait

GFP tag

kek1 
kek1 
kek1 
Egfr 

Egfr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kek1 
LRR domains
sufficient binding region
unspecified
kek1 
domain V residue 738
mutation decreasing interaction
aa 738

R738Q, SOK3 like mutation

kek1 
domain V residue 718
mutation decreasing interaction
aa 718

E718K, SOK4 like mutation

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S3 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Alvarado et al., 2004)
FBrf0222867-100.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kek1 

kek1

bait

Fc tag

kek1 
Egfr 

Egfr

prey

alkaline phosphatase tag, COMP pentamerization motif

Egfr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kek1 
extracellular domain
sufficient binding region
Egfr 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0222867-186.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Egfr 

Egfr

bait

Fc tag

Egfr 
kek1 

kek1

prey

alkaline phosphatase tag, COMP pentamerization motif

kek1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
extracellular domain
sufficient binding region
kek1 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)