FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
N, numb
FlyBase ID
FBig0000098592
Interaction Network
Interactions Browser links
N network
numb network
Reported Interactions
FBrf0089977-1.Y2H
Reference
Guo et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait

fused to LexA DNA-binding domain

N 
N 
numb 

Numb

prey

fused to GAL4 activation domain

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular RAM23 domain
sufficient binding region
aa 1766-1897
N 
C-terminal intracellular P domain
sufficient binding region
aa 2602-2703
numb 
N-terminal region
sufficient binding region
aa 1-330
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Guo et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Guo et al., 1996)
FBrf0089977-2.Y2H
Reference
Guo et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

fused to LexA DNA-binding domain

numb 
N 

Notch

prey

fused to GAL4 activation domain

N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
PTB domain
sufficient binding region
aa 1-223
N 
intracellular domain
sufficient binding region
aa 1792-2269
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Guo et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Guo et al., 1996)
FBrf0089977-3.PD.A
Reference
Guo et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait

GST tag

N 
numb 

Numb

prey

[35]S label

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1792-2703
numb 
PTB domain
sufficient binding region
aa 1-223
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Guo et al., 1996)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)