Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Bait was affinity purified by virtue of the His tag, and proteins were further purified by an anti-phosphotyrosine resin.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

SH3PX1 interacts strongly with the dock SH2 domain, and more weakly with the third dock SH3 domain.

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Purified SH3PX1 was phosphorylated by Ack in vitro and tested for binding to dock.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.