FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hpr1, thoc5
FlyBase ID
FBig0000098653
Interaction Network
Interactions Browser links
Hpr1 network
thoc5 network
Reported Interactions
FBrf0179407-2.coIP.MS
Reference
Rehwinkel et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

HPR1

bait

TAP tag

thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tho2, Hpr1, thoc5, thoc6 and thoc7 form the core THO complex. tex may be present in substoichiometric amounts.

(Rehwinkel et al., 2004)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Rehwinkel et al., 2004)
FBrf0209575-12.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

HPR1

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0209575-14.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

THOC5

bait
Hpr1 

HPR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0211509-10.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

HPR1

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0211509-12.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

THOC5

bait
Hpr1 

HPR1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0211509-3.CH.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

HPR1

neutral component
Hpr1 

HPR1

neutral component
Hpr1 

HPR1

neutral component
thoc5 

THOC5

neutral component
thoc5 

THOC5

neutral component
thoc5 

THOC5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins derived from endogenous genes.

(Gurskiy et al., 2010)
FBrf0232523-66.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

Thoc5

bait
Hpr1 

Hpr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0232523-67.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

Hpr1

bait
thoc5 

Thoc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0240110-12.coIP.MS
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

Thoc5

bait

Venus tag

Hpr1 

Hpr1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2018)
FBrf0240110-9.coIP.MS
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hpr1 

Hpr1

bait

Venus tag

thoc5 

Thoc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zhang et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)