Interaction in vitro; bait derived from wild type embryonic nuclear extract; prey produced and labeled by in vitro translation.
Purified Brahma complex was resolved by SDS-PAGE, transferred to a blot and proteins were renatured. The blot was then incubated with radiolabeled prey protein.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.