FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Fmr1, RpL5
FlyBase ID
FBig0000098704
Interaction Network
Interactions Browser links
Fmr1 network
RpL5 network
Reported Interactions
FBrf0152006-11.PD.A
Reference
Ishizuka et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMR1

bait

GST tag

Fmr1 
RpL5 

L5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
ribosome-binding domain
sufficient binding region
aa 535-684

C-terminal 150 residues

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Ishizuka et al., 2002)

The interaction is not affected by RNaseA treatment.

(Ishizuka et al., 2002)
FBrf0152006-13.PD.WB
Reference
Ishizuka et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMR1

bait

GST tag

RpL5 

L5

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ishizuka et al., 2002)
FBrf0152006-3.coIP.MS
Reference
Ishizuka et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMR1

bait

TAP tag

RpL5 

L5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Ishizuka et al., 2002)
FBrf0152006-7.coIP.WB
Reference
Ishizuka et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

dFMR1

bait

TAP tag

RpL5 

L5

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction is not affected by RNaseA treatment.

(Ishizuka et al., 2002)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ishizuka et al., 2002)
FBrf0225732-1.CL
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

Fmr1

bait
Fmr1 
RpL5 

RpL5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fmr1 
interaction domain
sufficient binding region
aa 220-681

coordinates relative to Fmr1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey was derived from 80S ribosomal fraction from embryos of wild-type fly line.

(Chen et al., 2014)
FBrf0225732-2.EM
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Fmr1 

Fmr1

neutral component
Fmr1 

Fmr1

neutral component
Fmr1 

Fmr1

neutral component
RpL5 

RpL5

neutral component
RpL5 

RpL5

neutral component
RpL5 

RpL5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system and derived from 80S ribosomal fraction from embryos of wild-type fly line.

(Chen et al., 2014)

Structure is of Fmr1 bound to 80S ribosome, interaction with RpL5 inferred.

(Chen et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)