FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kra, shot
FlyBase ID
FBig0000098740
Interaction Network
Interactions Browser links
kra network
shot network
Reported Interactions
FBrf0201378-10.coIP.WB
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
shot 

Shot

bait

GFP tag

kra 

Kra

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lee et al., 2007)
FBrf0201378-1a.coIP.WB
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
shot 

ShotL

bait

GFP tag

shot 
shot 
shot 
kra 

Kra

prey

HA tag

kra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
C-terminal region
sufficient binding region
aa 4689-5201
shot 
EF-hand motif
necessary binding region
shot 
GAS2 motif
necessary binding region

deletion weakens binding

kra 
W2 domain AA-box 2
mutation disrupting interaction
aa 401-411

7 residues mutated to alanine, \'7A\' mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lee et al., 2007)
FBrf0201378-4.PD.A
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kra 

Kra

bait

GST tag

shot 

Shot

prey

[35]S label

shot 
shot 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
EF-hand motif
sufficient binding region
aa 4688-4858
shot 
GAS2 motif
sufficient binding region
aa 4858-4984

weak binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lee et al., 2007)
FBrf0201378-8a.Y2H
Reference
Lee et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shot 

Shot

bait
shot 
kra 

kra

prey
kra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shot 
C-terminal region
sufficient binding region
aa 4688-5201
kra 
C-terminal region
sufficient binding region
aa 315-422
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Lee et al., 2007)

Two-hybrid system: yeast

(Lee et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)