Candidate cell surface proteins were screened for binding to an alkaline phosphatase tagged construct encoding the Ptp10D extracellular domain. 976 candidate genes encoding predicted cell surface proteins were identified, of which 311 genes had stocks available with upstream UAS insertions. Each of these 311 genes was ectopically expressed in pan-muscle or pan-neuronal patterns using the GAL4-UAS system, and live embryos were dissected and incubated with AP-tagged Ptp10D.

Source was live embryos of transgenic fly line; bait produced from tagged transgenic construct; prey derived from baculovirus-infected insect cells.

Ptp10D was overexpressed in the central nervous system, and live embryos were dissected and incubated with purified, Fc-tagged sas.

Source was live embryos of transgenic fly line; bait produced from tagged transgenic construct; prey derived from baculovirus-infected insect cells.

S2 cells expressing Ptp10D were mixed with S2 cells expressing sas, and the formation of cell clusters containing both Ptp10D and sas expressing cells was assayed by immunofluorescence.

Source was intact S2 cells; bait produced from transfected construct; prey produced from transfected construct.