FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Acf, His3
FlyBase ID
FBig0000098858
Interaction Network
Interactions Browser links
Acf network
His3 network
Reported Interactions
FBrf0182491-5.PD.MW
Reference
Eberharter et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Acf 

ACF1

bait

GST tag

Acf 
Acf 
His3 

H3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Acf 
PHD domains 1 and 2
sufficient binding region
aa 1039-1290
Acf 
bromodomain
sufficient binding region
aa 1300-1476
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Eberharter et al., 2004)

GST-tagged Acf1 was incubated with His2A, His2B, His3 and His4 simultaneously.

(Eberharter et al., 2004)
FBrf0182491-9.PD.MW
Reference
Eberharter et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Acf 

ACF1

bait

GST tag

Acf 
Acf 
His3 

H3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Acf 
PHD domains 1 and 2
sufficient binding region
aa 1039-1290
Acf 
bromodomain
sufficient binding region
aa 1300-1476
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Eberharter et al., 2004)

GST-tagged Acf1 was incubated with His3/His4 tetramers.

(Eberharter et al., 2004)
External Crossreferences and Linkouts ( 0 )
References (1)