FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
veli, sdt
FlyBase ID
FBig0000098981
Interaction Network
Interactions Browser links
veli network
sdt network
Reported Interactions
FBrf0174808-1.Y2H
Reference
Bachmann et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
veli 

DLin-7

bait

fused to GAL4 DNA-binding domain

sdt 

Sdt

prey

fused to GAL4 activation domain

sdt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sdt 
L27 domain
sufficient binding region
aa 641-834

1289 residue \'MAGUK1\' isoform tested

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bachmann et al., 2004)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bachmann et al., 2004)

veli binds sdt and CASK more strongly than dlg1 in this assay.

(Bachmann et al., 2004)
FBrf0174808-4.PD.TV
Reference
Bachmann et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
veli 

Dlin-7

bait

GST tag

sdt 

Sdt

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; produced and labeled by in vitro translation.

(Bachmann et al., 2004)
FBrf0174808-5.coIP.WB
Reference
Bachmann et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
veli 

Lin-7

bait
sdt 

Sdt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Drosophila veli was immunoprecipitated with an antibody raised against the mammalian ortholog.

(Bachmann et al., 2004)

veli coimmunoprecipitates sdt from embryos overexpressing sdt.

(Bachmann et al., 2004)

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Bachmann et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)