FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
rod, Zw10
FlyBase ID
FBig0000098987
Interaction Network
Interactions Browser links
rod network
Zw10 network
Reported Interactions
FBrf0139719-1.coIP.WB
Reference
Scaerou et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

DmROD

bait
Zw10 

DmZW10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Scaerou et al., 2001)
FBrf0139719-2.coIP.WB
Reference
Scaerou et al., 2001
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

DmROD

bait
Zw10 

DmZW10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Scaerou et al., 2001)
FBrf0139719-3.coIP.WB
Reference
Scaerou et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
rod 

DmROD

bait
Zw10 

DmZW10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Scaerou et al., 2001)
FBrf0139719-4.coIP.WB
Reference
Scaerou et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

DmROD

neutral component
rod 

DmROD

neutral component
rod 

DmROD

neutral component
Zw10 

DmZW10

neutral component
Zw10 

DmZW10

neutral component
Zw10 

DmZW10

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

rod and mit(1)15 cofractionate on gel filtration columns within a complex having an apparent molecular weight of 700-900 kDa.

(Scaerou et al., 2001)

Source was embryos of wild-type fly line; proteins produced from endogenous genes.

(Scaerou et al., 2001)
FBrf0159187-2.coIP.MS
Reference
Williams et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

ZW10

bait
rod 

ROD

prey

250kDa band

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Williams et al., 2003)

Zw10, rod, Zwilch and Hsc70-4 copurify in roughly equal stoichiometries.

(Williams et al., 2003)
FBrf0159187-5.coIP.WB
Reference
Williams et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

ZW10

bait
rod 

ROD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Williams et al., 2003)
FBrf0192076-5.coIP.MS
Reference
Chen et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

Protein A tag

rod 

Rod

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2007)

Positive control.

(Chen et al., 2007)
FBrf0208173-1.coIP.MS
Reference
D'Avino et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

Protein A tag

rod 

Rod

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(D'Avino et al., 2009)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(D'Avino et al., 2009)
FBrf0227800-1.coIP.WB
Reference
Défachelles et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 4embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 3embryonic cycle 4embryonic cycle 9embryonic stage 2
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

Rod

bait
Zw10 

Zw10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Défachelles et al., 2015)
FBrf0247450-1.coIP.WB
Reference
Menant and Karess, 2020
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

GFP tag

rod 

Rod

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was brains of transgenic larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Menant and Karess, 2020)
FBrf0247450-4.coIP.WB
Reference
Menant and Karess, 2020
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

Rod

bait

RFP tag

Zw10 

Zw10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was brains of transgenic larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Menant and Karess, 2020)
FBrf0247450-5.CM.MW
Reference
Menant and Karess, 2020
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rod 

Rod

neutral component

RFP tag

rod 

Rod

neutral component

RFP tag

rod 

Rod

neutral component

RFP tag

Zw10 

Zw10

neutral component
Zw10 

Zw10

neutral component
Zw10 

Zw10

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was brains of transgenic larvae; proteins produced from tagged transgenic construct or endogenous gene.

(Menant and Karess, 2020)

Ternary rod-Zw10-Zwilch complex captured as 3 binary interactions. Detection of the ternary complex requires a rod and Zw10 wild-type background.

(Menant and Karess, 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)