inaD, trp Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

[35]S label

inaD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1257-1275

coordinates relative to trp-PA

trp

C-terminal region

necessary binding region

aa 1267-1275

coordinates relative to trp-PA

inaD

central region

mutation disrupting interaction

aa 442

M442K, coordinates relative to inaD-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Shieh and Zhu, 1996)

FBrf0087705-2.FW.A

Reference

Shieh and Zhu, 1996

Description

physical association

Assay

far western blotting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

bait

GST tag

inaD

trp

TRP

prey

[35]S label

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1257-1275

coordinates relative to trp-PA

inaD

central region

sufficient binding region

aa 347-450

coordinates relative to inaD-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Shieh and Zhu, 1996)

FBrf0096480-1.coIP.WB

Reference

Tsunoda et al., 1997

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

bait

trp

TRP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was membranes from adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsunoda et al., 1997)

trp, norpA and inaC, but not ninaE or Gαq, coimmunoprecipitate with inaD.

(Tsunoda et al., 1997)

FBrf0096480-4.PD.WB

Reference

Tsunoda et al., 1997

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

bait

GST tag

inaD

trp

TRP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ3 domain

sufficient binding region

aa 362-449

coordinates relative to inaD-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Tsunoda et al., 1997)

FBrf0108298-4.coIP.WB

Reference

Wes et al., 1999

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

bait

trp

TRP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult heads from wild type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wes et al., 1999)

FBrf0125268-4.coIP.MW

Reference

Bahner et al., 2000

Description

physical association

Assay

molecular weight estimation by staining

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

inaD

INAD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult head membrane extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Bahner et al., 2000)

FBrf0129943-1.PD.A

Reference

Li and Montell, 2000

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1247-1275

coordinates relative to trp-PA

trp

C-terminal region

necessary binding region

aa 1272-1275

"Δ1272" construct, coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Li and Montell, 2000)

FBrf0129943-2.PD.WB

Reference

Li and Montell, 2000

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

inaD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1247-1275

coordinates relative to trp-PA

trp

C-terminal region

necessary binding region

aa 1272-1275

"Δ"1272 construct, coordinates relative to trp-PA

trp

C-terminal region residue V1266

mutation decreasing interaction

aa 1266

V1266D, coordinates relative to trp-PA

inaD

PDZ3 domain

mutation disrupting interaction

aa 442

M442K, coordinates relative to inaD-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult head extract.

(Li and Montell, 2000)

FBrf0204232-1.FW.A

Reference

Peng et al., 2008

Description

physical association

Assay

far western blotting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1262-1275

coordinates relative to trp-PA

trp

C-terminal region residue K1263

mutation decreasing interaction

aa 1263

K1263A or K1263D, coordinates relative to trp-PA

trp

C-terminal region residue S1264

mutation disrupting interaction

aa 1264

S1264T, coordinates relative to trp-PA

trp

C-terminal region residue T1265

mutation decreasing interaction

aa 1265

T1265P, coordinates relative to trp-PA

trp

C-terminal region residue V1266

mutation disrupting interaction

aa 1266

V1266D or V1266A, coordinates relative to trp-PA

trp

C-terminal region residue G1262

mutation decreasing interaction

aa 1262

G1262A or G1262D, coordinates relative to trp-PA

trp

C-terminal region residue W1274

mutation disrupting interaction

aa 1274

W1274D or W1274A, coordinates relative to trp-PA

trp

C-terminal region residue L1275

mutation disrupting interaction

aa 1275

L1275D or L1275A, coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Peng et al., 2008)

FBrf0204232-2.PD.WB

Reference

Peng et al., 2008

Description

physical association

Assay

fluorescence polarization spectroscopy

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

trp

C-terminal region

sufficient binding region

aa 1262-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from adult head extract.

(Peng et al., 2008)

FBrf0214003-3.FPS

Reference

Liu et al., 2011

Description

physical association

Assay

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

bait

inaD

trp

Trp

prey

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ5 domain

sufficient binding region

aa 580-665

coordinates relative to inaD-PA

trp

region

sufficient binding region

TGRMISGWL

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

(Liu et al., 2011)

The inaD PDZ5 domain binds trp peptide with Kd = 140uM (relatively weak).

(Liu et al., 2011)

FBrf0214003-4.NMR

Reference

Liu et al., 2011

Description

physical association

Assay

nuclear magnetic resonance

predetermined participant

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

neutral component

inaD

INAD

neutral component

inaD

INAD

neutral component

inaD

trp

TRP

neutral component

trp

TRP

neutral component

trp

TRP

neutral component

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ45 domain

sufficient binding region

aa 473-674

coordinates relative to inaD-PA

trp

C-terminal region

sufficient binding region

aa 1261-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

(Liu et al., 2011)

inaD binds two discrete sites in the trp C-terminal tail. The inaD PDZ3 domain binds to the trp C-terminal STV sequence (aa 1264-1266) and inaD PDZ4-5 domains bind to the trp C-terminal GWL sequence (aa 1273-1275).

(Liu et al., 2011)

FBrf0222115-3.coIP.WB

Reference

Katz et al., 2013

Description

physical association

Assay

anti tag coimmunoprecipitation

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GFP tag

inaD

INAD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Transgenic trp expressed in photoreceptor cells. Anti-GFP antibody used for immunoprecipitation.

(Katz et al., 2013)

The interaction of GFP-tagged trp with inaD is not observed in a trp⁹ mutant background. The interpretation is that the GFP tag disrupts direct trp-inaD interaction, but that GFP-trp exists in heteromultimers with wild-type trp proteins that do interact with inaD.

(Katz et al., 2013)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Katz et al., 2013)

FBrf0222115-4.coIP.WB

Reference

Katz et al., 2013

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

inaD

INAD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Katz et al., 2013)

FBrf0229940-2.PD.WB

Reference

Chen et al., 2015

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

SBP tag

inaD

INAD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chen et al., 2015)

SBP-tagged proteins pulled down using streptavidin beads.

(Chen et al., 2015)

Positive control.

(Chen et al., 2015)

FBrf0231190-1.PD.CH

Reference

Ye et al., 2016

Description

physical association

Assay

nuclear magnetic resonance

molecular sieving

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

bait

His tag

inaD

trp

TRP

prey

GB1 tag

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ3

sufficient binding region

aa 351-448

coordinates relative to inaD-PA

trp

C-terminal region

sufficient binding region

aa 1261-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2016)

inaD and trp were co-expressed in bacterial cells and purified by His-tag affinity and size exclusion to obtain a complex for further analysis.

(Ye et al., 2016)

FBrf0231190-2.NMR

Reference

Ye et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

neutral component

His tag

inaD

INAD

neutral component

His tag

inaD

INAD

neutral component

His tag

inaD

trp

TRP

neutral component

trp

TRP

neutral component

trp

TRP

neutral component

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ3

sufficient binding region

aa 351-448

coordinates relative to inaD-PA

trp

C-terminal region

sufficient binding region

aa 1261-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2016)

inaD and trp were co-expressed in bacterial cells and purified by His-tag affinity and size exclusion to obtain a complex for further analysis.

(Ye et al., 2016)

FBrf0231190-3.CS

Reference

Ye et al., 2016

Description

physical association

Assay

cosedimentation

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

inaD

INAD

neutral component

His tag

inaD

INAD

neutral component

His tag

inaD

INAD

neutral component

His tag

inaD

trp

TRP

neutral component

trp

TRP

neutral component

trp

TRP

neutral component

trp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ3

sufficient binding region

aa 351-448

coordinates relative to inaD-PA

trp

C-terminal region

sufficient binding region

aa 1261-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2016)

inaD PDZ3 domain binds the trp C-terminal region with Kd = 0.13uM.

(Ye et al., 2016)

inaD and trp were co-expressed in bacterial cells and purified by His-tag affinity and size exclusion to obtain a complex for further analysis.

(Ye et al., 2016)

FBrf0231190-4.PD.MW

Reference

Ye et al., 2016

Description

physical association

Assay

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

TRP

bait

GST tag

trp

inaD

INAD

prey

His tag

inaD

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

inaD

PDZ3

sufficient binding region

aa 351-448

coordinates relative to inaD-PA

inaD

PDZ3 residue M442

mutation decreasing interaction

aa 442

M442K, coordinates relative to inaD-PA

trp

C-terminal region

sufficient binding region

aa 1261-1275

coordinates relative to trp-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Ye et al., 2016)

FBrf0235287-1.coIP.WB

Reference

Voolstra et al., 2017

Description

physical association

Assay

Collection

Source/Stage

adult head / adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

trp

bait

inaD

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Voolstra et al., 2017)

FBrf0246134-4.PD.MS

Reference

Chen and Montell, 2020

Description

physical association

Assay