FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Zw10, Zwilch
FlyBase ID
FBig0000099001
Interaction Network
Interactions Browser links
Zw10 network
Zwilch network
Reported Interactions
FBrf0159187-3.coIP.MS
Reference
Williams et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

ZW10

bait
Zwilch 

Zwilch

prey

75kDa band

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Williams et al., 2003)

Zw10, rod, Zwilch and Hsc70-4 copurify in roughly equal stoichiometries.

(Williams et al., 2003)
FBrf0159187-6.coIP.WB
Reference
Williams et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

ZW10

bait
Zwilch 

Zwilch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Williams et al., 2003)
FBrf0159187-7.coIP.MS
Reference
Williams et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zwilch 

Zwilch

bait
Zw10 

ZW10

prey

85kDa band

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Zw10, rod, Zwilch and Hsc70-4 copurify in roughly equal stoichiometries.

(Williams et al., 2003)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Williams et al., 2003)
FBrf0192076-6.coIP.MS
Reference
Chen et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

Protein A tag

Zwilch 

Zwilch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Chen et al., 2007)

Positive control.

(Chen et al., 2007)
FBrf0208173-2.coIP.MS
Reference
D'Avino et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

Protein A tag

Zwilch 

Zwilch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(D'Avino et al., 2009)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(D'Avino et al., 2009)
FBrf0247450-2.coIP.WB
Reference
Menant and Karess, 2020
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zw10 

Zw10

bait

GFP tag

Zwilch 

Zwilch

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was brains of transgenic larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Menant and Karess, 2020)
FBrf0247450-7.CM.MW
Reference
Menant and Karess, 2020
Description
physical association
Assay
Collection
Source/Stage
brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zwilch 

Zwilch

neutral component
Zwilch 

Zwilch

neutral component
Zwilch 

Zwilch

neutral component
Zw10 

Zw10

neutral component
Zw10 

Zw10

neutral component
Zw10 

Zw10

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was brains of transgenic larvae; proteins produced from tagged transgenic construct or endogenous gene.

(Menant and Karess, 2020)

Ternary rod-Zw10-Zwilch complex captured as 3 binary interactions. Detection of the ternary complex requires a rod and Zw10 wild-type background.

(Menant and Karess, 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)