Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide synthesized in vitro.

Scm binds to His4 peptides monomethlyated at K20. Binding to unmodified, di- or trimethylated peptides was too weak to determine a binding constant.

Interaction in vitro; His4 peptide synthesized in vitro; Sfmbt produced as a recombinant fusion protein in bacterial system.

The methyl-lysine side chain occupies a binding pocket in the second MBT domain formed by three aromatic residues and one aspartate.

Scm carried a R277C mutation to facilitate crystallization.