FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gαi, Gβ13F
FlyBase ID
FBig0000099028
Interaction Network
Interactions Browser links
Gαi network
Gβ13F network
Reported Interactions
FBrf0139609-3.coIP.WB
Reference
Schaefer et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαi 

Gαi

bait
Gβ13F 

Gβ

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The addition of recombinant raps (Pins), or a raps peptide (aa 601-638) inhibits the coimmunoprecipitation of Gβ13F with Gαi. This suggests that binding of Gαi to Gα13F or raps is mutually exclusive.

(Schaefer et al., 2001)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Schaefer et al., 2001)
FBrf0191262-4.coIP.WB
Reference
Hampoelz et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Gαi 

Gαi

bait
Gβ13F 

Gβ13F

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hampoelz et al., 2005)

Gβ13F does not interact with Gαi if Gαi is bound to GTP.

(Hampoelz et al., 2005)
FBrf0191262-8.coIP.WB
Reference
Hampoelz et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαi 

Gαi

bait
Gβ13F 

Gβ13F

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryoss of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hampoelz et al., 2005)

Similar experiments done in a ric8a mutant background demonstrated the requirement of ric8a for interaction between Gβ13F and Gαi.

(Hampoelz et al., 2005)
FBrf0191270-4.coIP.WB
Reference
Wang et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαi 

Gαi

bait
Gβ13F 

Gβ13F

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wang et al., 2005)

In this experiment, Gβ13F dos not interact with GTP bound Gαi.

(Wang et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)