Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

Interaction in vitro; bait produced by in vitro transcription; prey produced and labeled by in vitro translation.

egl binds to the fs(1)K10 TLS localization signal directly, despite lacking a canonical RNA-binding motif. Neither BicD nor dynein light chain bind RNA directly in this assay.

Mutation of two residues essential for putative exonuclease activity has no effect on binding of egl to fs(1)K10 TLS RNA.

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

Interaction in vitro; bait produced by in vitro transcription; prey produced as a recombinant fusion protein in yeast system.

Recombinant egl, or an egl-BicD complex, can bind the fs(1)K10 TLS.

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

Interaction in vitro; bait produced as a recombinant fusion protein in yeast system; prey produced and labeled by in vitro transcription.

A recombinant egl-BicD complex binds the fs(1)K10 TLS.

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

BicD and egl were the only proteins pulled down consistently and specifically with wild-type localization signals from fs(1)K10 (TLS), grk (GLS), h (SL1) and I-element (ILS).

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).

egl and BicD are able to interact with RNA localization signals in the absence of Lis-1.

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced and labeled by in vitro translation.

Positive control.

egl binds to the fs(1)K10 TLS localization signal directly.

Endogenous egl was depleted with shRNA. Bait RNA was pulled down by an RNA aptamer tag with high affinity for streptavidin beads. Introduction of a yeast leucine zipper to restore dimerization of egl 2pt mutant artificially, restores interaction with fs(1)K10.

Interaction in vitro; bait produced by in vitro transcription; prey derived from transgenic adult female ovary extract.