FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hry, egl
FlyBase ID
FBig0000099104
Interaction Network
Interactions Browser links
hry network
egl network
Reported Interactions
FBrf0208220-13.PD.WB
Reference
Dienstbier et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

h

bait

SA tag

hry 
hry 
egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
SL1
sufficient binding region
CAUUUCACAUCAUUCGCCGGGAUUGCGCAAAUGUUGCUUUGAAGUG
hry 
SL1
mutation disrupting interaction
nt 15

C15G

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Dienstbier et al., 2009)

BicD and egl were the only proteins pulled down consistently and specifically with wild-type localization signals from fs(1)K10 (TLS), grk (GLS), h (SL1) and I-element (ILS).

(Dienstbier et al., 2009)

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

(Dienstbier et al., 2009)
FBrf0222317-12.PD.MS
Reference
Dix et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

hairy

bait

TRAP tag

hry 
hry 
egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
HLE
sufficient binding region
CAUUUCACAUCAUUCGCCGGGAUUGCGCAAAUGUUGCUUUGAAGUGUUGCAAACAUGCGAAUCCUAAACUCGGUUCACAACUUCGUUGGCUUAGUUUCCUGGCUUAUAUCCUGGAAACCCGUCGAC
hry 
HLE-SL1
mutation disrupting interaction
nt 41-43

GAA-to-CUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).

(Dix et al., 2013)

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Dix et al., 2013)
FBrf0222317-27.PD.WB
Reference
Dix et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

hairy

bait

TRAP tag

hry 
hry 
egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
HLE
sufficient binding region
CAUUUCACAUCAUUCGCCGGGAUUGCGCAAAUGUUGCUUUGAAGUGUUGCAAACAUGCGAAUCCUAAACUCGGUUCACAACUUCGUUGGCUUAGUUUCCUGGCUUAUAUCCUGGAAACCCGUCGAC
hry 
HLE-SL1
mutation disrupting interaction
nt 41-43

GAA-to-CUU

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads. Less stringent wash conditions were used in these purifications (compared to a previous study, FBrf0208220).

(Dix et al., 2013)

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Dix et al., 2013)
FBrf0224776-7.coIP.PCR
Reference
Vazquez-Pianzola et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait
hry 

h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)
FBrf0234579-31.coIP.SEQ
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

hry 

h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0234579-4.coIP.PCR
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

hry 

h

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)