Source was embryos of wild-type fly line; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

N binds cno but not dsh in this in vitro assay.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

N binds cno but not dsh in this two hybrid assay.

cno interacts with two different regions of the N intracellular domain.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

N, cno and Ser colocalize at the apical side of neuroepithelial cells, at the interface with surrounding glia.