FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
osk, heph
FlyBase ID
FBig0000099248
Interaction Network
Interactions Browser links
osk network
heph network
Reported Interactions
FBrf0206986-1.coIP.PCR
Reference
Besse et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
heph 

PTB

bait

GFP tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Besse et al., 2009)
FBrf0206986-2.PD.WB
Reference
Besse et al., 2009
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

biotin label

osk 
osk 
heph 

PTB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR
sufficient binding region
nt 1834-2890
osk 
M1M2
sufficient binding region
nt 1-434
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovarian extract.

(Besse et al., 2009)
FBrf0206986-4.EMSA.A
Reference
Besse et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
heph 

PTB

bait

MBP tag

osk 

osk

prey

ATTO-680 label

osk 
osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
M1M2
sufficient binding region
unspecified
osk 
3-prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Besse et al., 2009)

Two complexes form when osk M1M2 RNA is incubated with increasing amounts of recombinant heph protein, while one higher complex forms with osk 3-prime UTR RNA.

(Besse et al., 2009)
FBrf0222280-3.coIP.PCR
Reference
McDermott and Davis, 2013
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
heph 

Heph

bait

GFP tag

osk 

oskar

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

heph interacts with grk and osk RNA, but not bcd, nos or hb RNA.

(McDermott and Davis, 2013)

Source was adult female ovary of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(McDermott and Davis, 2013)
FBrf0232132-1a.XL.A
Reference
Macdonald et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

[32]P label

osk 
heph 

PTB

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Macdonald et al., 2016)
FBrf0253176-3.EMSA
Reference
Bose et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
heph 

PTB

bait

His-RFP tag

osk 

oskar

prey

atto633 label

osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3 prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in Sf-21 cell system; prey produced and labeled by in vitro transcription.

(Bose et al., 2022)
FBrf0260912-3.PD.WB
Reference
Bose et al., 2024
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

biotin label

osk 
heph 

PTB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR
sufficient binding region
nt 1-359
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovary extract.

(Bose et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)