Antibodies used for EcR detection recognized the domain commmon to all isoforms.

Source was third instar larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Kdm4A and EcR also colocalize on salivary gland polytene chromosomes.

Adding ecdysone to cultured S2 cells increased the amount of both Kdm4A and Kdm4B.