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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099282
Interaction Network
Interactions Browser links
msl-2 network
lncRNA:roX1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204458-1.coIP.PCR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MSL2

bait

roX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 1-743
interaction domain
necessary binding region
aa 684-743
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type adult female flies; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0222154-7.XL.coIP.SEQ
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MSL2

bait

roX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
region D1
sufficient binding region
nt 966-1550

coordinates relative to transcript roX1-RC

region D2
sufficient binding region
nt 2340-2827

coordinates relative to transcript roX1-RC

region D3
sufficient binding region
nt 3394-3843

coordinates relative to transcript roX1-RC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

RNA-protein interactions were characterized using the iCLIP protcol (cross-linking and immunoprecipitation). RNA was UV-crosslinked to protein, isolated by immunoprecipitation using msl-2 antibody, and characterized by deep sequencing analysis.

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Though msl-2 bound to 5,206 sites, roX1 and roX2 sites scored considerably higher, representing over half of all observed cross-linking events.

FBrf0240007-1.CLIP.PCR
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

MSL2

bait

HA tag

roX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)