FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
msl-2, lncRNA:roX1
FlyBase ID
FBig0000099282
Interaction Network
Interactions Browser links
msl-2 network
lncRNA:roX1 network
Reported Interactions
FBrf0204458-1.coIP.PCR
Reference
Li et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-2 

MSL2

bait
msl-2 
msl-2 
lncRNA:roX1 

roX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-2 
interaction domain
sufficient binding region
aa 1-743
msl-2 
interaction domain
necessary binding region
aa 684-743
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type adult female flies; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2008)
FBrf0222154-7.XL.coIP.SEQ
Reference
Ilik et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
msl-2 

MSL2

bait
lncRNA:roX1 

roX1

prey
lncRNA:roX1 
lncRNA:roX1 
lncRNA:roX1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lncRNA:roX1 
region D1
sufficient binding region
nt 966-1550

coordinates relative to transcript roX1-RC

lncRNA:roX1 
region D2
sufficient binding region
nt 2340-2827

coordinates relative to transcript roX1-RC

lncRNA:roX1 
region D3
sufficient binding region
nt 3394-3843

coordinates relative to transcript roX1-RC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

RNA-protein interactions were characterized using the iCLIP protcol (cross-linking and immunoprecipitation). RNA was UV-crosslinked to protein, isolated by immunoprecipitation using msl-2 antibody, and characterized by deep sequencing analysis.

(Ilik et al., 2013)

Source was cell extract of clone8 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ilik et al., 2013)

Though msl-2 bound to 5,206 sites, roX1 and roX2 sites scored considerably higher, representing over half of all observed cross-linking events.

(Ilik et al., 2013)
FBrf0240007-1.CLIP.PCR
Reference
Valsecchi et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / wandering third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
msl-2 

MSL2

bait

HA tag

lncRNA:roX1 

roX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Valsecchi et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)