14-3-3ε
GST tag
Gwl
Myc tag
K49E
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.
Treatment of cells with the cdc2 inhibitor RO-3306 does not inhibit the interaction of gwl and 14-3-3ε.
Protein A tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Mutation of CdkA sites in gwl does not disrupt the interaction with 14-3-3ε.
