FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Egfr, vap
FlyBase ID
FBig0000099414
Interaction Network
Interactions Browser links
Egfr network
vap network
Reported Interactions
FBrf0222949-2.coIP.WB
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait

FLAG tag

vap 

RasGAP

prey

HA tag

vap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2-SH3-SH2 domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bianco et al., 2007)
FBrf0222949-23.Y2H
Reference
Bianco et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait
Egfr 
Egfr 
vap 

RasGAP/Vap

prey
vap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Egfr 
intracellular domain
sufficient binding region
Egfr 
intracellular domain
mutation decreasing interaction
aa 1095-1342

all Tyr residues mutated to Phe in this region

vap 
SH2-SH3-SH2 domains
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Bianco et al., 2007)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bianco et al., 2007)
FBrf0225482-6.coIP.WB
Reference
Rowshanravan et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Egfr 

EGFR

bait

GFP tag

vap 

RasGAP

prey

Myc tag

vap 
vap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH2 domain 1
mutation decreasing interaction
aa 110

R110L

vap 
SH2 domain 2
mutation decreasing interaction
aa 278

R278L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rowshanravan et al., 2014)

Mutation of both vap SH2 domains abolishes the interaction with Egfr.

(Rowshanravan et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)