Exo70
fused to LexA DNA-binding domain
Arm
fused to GAL4 activation domain
Two-hybrid system: yeast LexA-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
arm binds sec10 and exo70, but not sec8 or exo84 in this assay.
The exo70-arm interaction could not be confirmed by GST pull down assay.