FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000099463
Interaction Network
Interactions Browser links
morgue network
Ubi-p5E network
Reported Interactions
FBrf0222885-1.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

morgue

bait

FLAG tag

Ubiquitin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The specific ubiquitin linkages were also determined by mass spectrometry.

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

morgue specifically associates with K48-linked ubiquitin multimers.

FBrf0222885-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

morgue

bait

FLAG tag

Ubiquitin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

morgue specifically associates with ubiquitin multimers.

FBrf0222885-7.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

morgue

bait

MBP tag

Ubiquitin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal region
sufficient binding region
aa 1-301
C-terminal region
sufficient binding region
aa 302-491
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein and polymerized in vitro.

Both the N-terminal and C-terminal regions of morgue bind polyubiquitin.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)