Smo
Myc tag
Vps36
V5 tag
\'Δ818\'
\'Δ661-818\'
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
smo interacts with Vps36, but not Vps22 or Vps25 in this assay.
Treatment of cells with hh disrupts the interaction of smo with Vps36.
Mutations in the smo C-tail that mimic the phosphorylated state disrupt binding by Vps36.
Cloning a ubiquitin polypeptide into the C-terminal end of smo yields the interaction with Vps36 resistant to hh-mediated disruption.
Cotransfection of cells with Ubpy (Usp8, a smo deubiquitinylase), disrupts the smo-Vps36 interaction.
