FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
grk, egl
FlyBase ID
FBig0000099508
Interaction Network
Interactions Browser links
grk network
egl network
Reported Interactions
FBrf0208220-15.PD.WB
Reference
Dienstbier et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grk 

grk

bait

SA tag

grk 
egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grk 
GLS
sufficient binding region
AGUAAUUUUCGUGCUCUCAACAAUUGUCGCCGUCACAGAUUGUUGUUCGAGCCGAAUCUUACU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from wild-type embryonic extract.

(Dienstbier et al., 2009)

BicD and egl were the only proteins pulled down consistently and specifically with wild-type localization signals from fs(1)K10 (TLS), grk (GLS), h (SL1) and I-element (ILS).

(Dienstbier et al., 2009)

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

(Dienstbier et al., 2009)
FBrf0208220-18.PD.WB
Reference
Dienstbier et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grk 

grk

bait

SA tag

grk 
egl 

Egl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grk 
GLS
sufficient binding region
AGUAAUUUUCGUGCUCUCAACAAUUGUCGCCGUCACAGAUUGUUGUUCGAGCCGAAUCUUACU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey produced as a recombinant fusion protein in yeast system.

(Dienstbier et al., 2009)

Recombinant egl, or an egl-BicD complex, can bind the grk GLS.

(Dienstbier et al., 2009)

Bait RNA was pulled down by virtue of an RNA aptamer tag with high affinity for streptavidin beads.

(Dienstbier et al., 2009)
FBrf0234579-30.coIP.SEQ
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

grk 

grk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0234579-7.coIP.PCR
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

grk 

grk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0243139-9.coIP.PCR
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

RFP tag

egl 
egl 
grk 

grk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation decreasing interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

egl 
N-terminal domain residue 35
mutation decreasing interaction
aa 35

C35Y, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries from transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2019)
FBrf0250118-4a.PD.WB
Reference
Neiswender et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grk 

grk

bait

SA tag

grk 
egl 

Egl

prey

FLAG tag

egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grk 
GLS
sufficient binding region
AGUAAUUUUCGUGCUCUCAACAAUUGUCGCCGUCACAGAUUGUUGUUCGAGCCGAAUCUUACU
egl 
2pt interaction domain residues 965 and 969
mutation decreasing interaction
aa 965 and 969

S965K and S969R; coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Endogenous egl was depleted with shRNA. Bait RNA was pulled down by an RNA aptamer tag with high affinity for streptavidin beads. Introduction of a yeast leucine zipper to restore dimerization of egl 2pt mutant artificially, restores interaction with grk.

(Neiswender et al., 2021)

egl binds to the grk GLS localization signal directly.

(Neiswender et al., 2021)

Interaction in vitro; bait produced by in vitro transcription; prey derived from transgenic adult female ovary extract.

(Neiswender et al., 2021)
FBrf0250118-5a.coIP.PCR
Reference
Neiswender et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

FLAG tag

egl 
grk 

grk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
2pt interaction domain residues 965 and 969
mutation decreasing interaction
aa 965 and 969

S965K and S969R; coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Endogenous egl was depleted with shRNA. Introduction of a yeast leucine zipper to restore dimerization of egl 2pt mutant artificially, partially restores interaction with grk.

(Neiswender et al., 2021)

Interaction in vitro; bait produced by in vitro transcription; prey derived from transgenic adult female ovary extract.

(Neiswender et al., 2021)
FBrf0251875-1.coIP.PCR
Reference
Goldman et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

FLAG tag

egl 
grk 

grk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
RNA binding domain residues 676-726
mutation decreasing interaction
nt 676-726

truncated RNA binding domain; coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)