FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arl1, GCC185
FlyBase ID
FBig0000099600
Interaction Network
Interactions Browser links
Arl1 network
GCC185 network
Reported Interactions
FBrf0206354-12.Y2H
Reference
Sinka et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl1 

Arl1

bait

fused to GAL4 DNA-binding domain

GCC185 

dGCC185

prey

fused to GAL4 activation domain

GCC185 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
GCC185 
C-terminal region
sufficient binding region
aa 900-1111
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sinka et al., 2008)

From a panel of 27 small G proteins tested, CG3532 interacted only with Arl1.

(Sinka et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sinka et al., 2008)
FBrf0225043-1.Y2H
Reference
Torres et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl1 

Arl1

bait

fused to GAL4 DNA-binding domain

GCC185 

dGCC185

prey

fused to GAL4 activation domain

GCC185 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
GCC185 
GRIP domain
sufficient binding region
aa 979-1135
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Torres et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Torres et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)