Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from testis cDNA expression library).

Two-hybrid system: yeast LexA-BD/VP16-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Topors was assayed for the ability to supershift a complex of h with [32]P labeled DNA (40-mer) from the ac promoter. Topors alone does not bind to the DNA probe.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Topors binds h, but not the related proteins dpn, E(spl)m3-HLH or E(spl)m8-HLH.