FlyBase Gene Report: Dmel\Topors

General Information

Symbol

Dmel\Topors

Species

D. melanogaster

Name

Topoisomerase I-interacting protein

Annotation Symbol

CG15104

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267351

Gene Model Status

Current

Stock Availability

15 publicly available

Enzyme Name (EC)

Gene Summary

Topoisomerase I-interacting protein (Topors) encodes a RING-finger containing putative dual Ubiquitin/SUMO ligase. It is required in the male germline for maintenance of nuclear shape and meiosis I chromosome segregation. It also targets ubiquitination of the developmental transcription factor encoded by hry. [Date last reviewed: 2024-07-18] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dTopors, nuclear blebber, nbl

Key Links

Genomic Location

Cytogenetic map

56A2-56A2

Sequence location

2R:18,972,612..18,977,262 [+]

Recombination map

2-87

RefSeq locus

NT_033778 REGION:18972612..18977262

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables lamin binding

inferred from direct assay

(Capelson and Corces, 2005)

enables protein binding

inferred from physical interaction with UniProtKB:P08970

(Capelson and Corces, 2005)

inferred from physical interaction with UniProtKB:Q86B87

(Capelson and Corces, 2005)

inferred from physical interaction with UniProtKB:P14003

(Secombe and Parkhurst, 2004)

enables ubiquitin protein ligase activity

inferred from direct assay

(Secombe and Parkhurst, 2004)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables metal ion binding

inferred from electronic annotation with InterPro:IPR018957

(InterPro Project Members, 2004-)

enables SUMO transferase activity

inferred from sequence or structural similarity with UniProtKB:Q9NS56

(Gene Ontology Curators, 2002-)

enables ubiquitin protein ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN002505744

(GO Reference Genome Project, 2011-)

enables zinc ion binding

inferred from sequence model

(Ying et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in centrosome separation

inferred from mutant phenotype

(Matsui et al., 2011)

involved_in male meiosis chromosome segregation

inferred from mutant phenotype

(Matsui et al., 2011)

involved_in meiotic chromosome condensation

inferred from mutant phenotype

(Matsui et al., 2011)

involved_in nuclear membrane organization

inferred from mutant phenotype

(Matsui et al., 2011)

involved_in nucleus organization

inferred from mutant phenotype

(Capelson and Corces, 2005)

involved_in protein polyubiquitination

inferred from direct assay

(Secombe and Parkhurst, 2004)

involved_in regulation of DNA-templated transcription

inferred from genetic interaction with UniProtKB:P14003

(Secombe and Parkhurst, 2004)

involved_in ubiquitin-dependent protein catabolic process

inferred from direct assay

(Secombe and Parkhurst, 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein monoubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN002505744

(GO Reference Genome Project, 2011-)

involved_in protein polyubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN002505744

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in nuclear lamina

inferred from direct assay

(Matsui et al., 2011, Capelson and Corces, 2005)

located_in nucleus

inferred from direct assay

(Capelson and Corces, 2005)

located_in polytene chromosome

inferred from direct assay

(Capelson and Corces, 2005)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

UNCLASSIFIED RING DOMAIN UBIQUITIN LIGASES

SUMO TRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ubiquitin protein ligase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED RING DOMAIN UBIQUITIN LIGASES -: This group comprises RING domain ubiquitin ligases that do not classify under other groups in FlyBase.
SUMO TRANSFERASES -: SUMO transferases catalyze the transfer of SUMO from either E2 or E3 enzyme to a substrate.

Protein Function (UniProtKB)

Functions as a ubiquitin-protein E3 ligase. Negatively regulates the transcriptional repressor hairy/hry by promoting its ubiquitination and subsequent degradation. Also directs the nuclear organization of the gypsy chromatin insulator. Chromatin insulators are regulatory elements which establish independent domains of transcriptional activity within eukaryotic genomes. Insulators have two defining properties; they can block the communication between an enhancer and a promoter when placed between them, and can also buffer transgenes from position effect variegation (PEV). Insulators are proposed to structure the chromatin fiber into independent domains of differing transcriptional potential by promoting the formation of distinct chromatin loops. This chromatin looping may require the formation of insulator bodies, where homotypic interactions between individual subunits of the insulator complex could promote the clustering of widely spaced insulators at the nuclear periphery. Within the gypsy insulator complex, this protein may promote formation of nuclear insulator bodies by recruiting individual insulator complexes to the nuclear lamina.

(UniProt, Q9V8P9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Topors for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V8P9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Topors-RA

FBtr0086570

NM_137544

4042

1038

Topors-RB

FBtr0331977

NM_001274154

4298

1038

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Topors-PA

FBpp0085754

113.3

1038

4.38

FBpp0304302

113.3

1038

4.38

Q9V8P9

NP_001261083

AGB93615

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1038 aa isoforms: Topors-PA, Topors-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with hairy/hry, p53 and Top1. Interacts with the gypsy chromatin insulator complex, composed of Cp190, mod(mdg4) and su(Hw); interacts directly with mod(mdg4) and su(Hw). Interacts with Lam/lamin.

(UniProt, Q9V8P9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Topors using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.24

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nuclear lamina

inferred from direct assay

(Matsui et al., 2011, Capelson and Corces, 2005)

located_in nucleus

inferred from direct assay

(Capelson and Corces, 2005)

located_in polytene chromosome

inferred from direct assay

(Capelson and Corces, 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Topors in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Topors

View exonic expression by tissue for Dmel\Topors

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37188/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267351

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034410

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267351

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15104

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Topors

Transgenic constructs containing regulatory region of Topors

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene partially disrupted in

Df(2R)Topors^AA

(Secombe and Parkhurst, 2004)

Gene disrupted in

Df(2R)Exel6068

(Matsui et al., 2011, Ryder, 2004.4.26)

Df(2R)PC66

(Matsui et al., 2011)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Topors^AA

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Topors^GD4575

viable, with Scer\GAL4^Mef2.PR

Topors^GD4575

viable, with Scer\GAL4^pnr-MD237

Topors^GD4575

viable, with Scer\GAL4^tin.CΔ4

Topors^GD4575

Sterility

Allele

male fertile

male semi-sterile (with Df(2R)Topors^AA)

male semi-sterile (with Topors^f05115)

male semi-sterile (with Topors^Z1837)

male semi-sterile (with Topors^Z4522)

male semi-sterile | recessive

male sterile

Topors^Z1837

Other Phenotypes

Allele

abnormal meiotic cell cycle | dominant | male limited

Topors^Z1837

abnormal meiotic cell cycle | male limited (with Df(2R)Topors^AA)

abnormal meiotic cell cycle | male limited (with Topors^f05115)

abnormal meiotic cell cycle | male limited (with Topors^Z1837)

abnormal meiotic cell cycle | male limited (with Topors^Z4522)

abnormal meiotic cell cycle | male limited | recessive

decreased fecundity | male

decreased fecundity | male (with Df(2R)Topors^AA)

decreased fecundity | male (with Topors^f05115)

decreased fecundity | male (with Topors^Z1837)

decreased fecundity | male (with Topors^Z4522)

Phenotype manifest in

Allele

centriole | male limited

Topors^f05115

nucleus

spermatocyte

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (35)

Hsap\TOPORS

10 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RNF126

1 of 14

Hsap\RNF139

1 of 14

Hsap\RNF141

1 of 14

Yes

Hsap\RNF145

1 of 14

Hsap\RNF170

1 of 14

Yes

Hsap\RNF185

1 of 14

Hsap\RNF213

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TRIM26

1 of 14

Yes

Hsap\TRIM31

1 of 14

Yes

Hsap\TRIM39

1 of 14

Yes

Hsap\TRIM65

1 of 14

Yes

Hsap\TRIM68

1 of 14

Hsap\ZNRF4

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (29)

Rnor\Topors

10 of 14

Yes

Rnor\Pex10

2 of 14

Rnor\Amfr

1 of 14

Rnor\Dtx3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Rlim

1 of 14

Rnor\Rnf5

1 of 14

Rnor\Rnf114

1 of 14

Yes

Rnor\Rnf139

1 of 14

Rnor\rnf141

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Trim31

1 of 14

Yes

Rnor\Trim50

1 of 14

Yes

Rnor\Trim65

1 of 14

Yes

Rnor\Trim68

1 of 14

Mus musculus (laboratory mouse) (33)

Mmus\Topors

10 of 14

Yes

Mmus\Toporsl

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Dtx3

1 of 14

Yes

Mmus\Dzip3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Rnf114

1 of 14

Yes

Mmus\Rnf139

1 of 14

Mmus\Rnf141

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Trim40

1 of 14

Yes

Mmus\Trim47

1 of 14

Mmus\Trim65

1 of 14

Yes

Mmus\Trim68

1 of 14

Mmus\Zswim2

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (25)

Xtro\topors

6 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\dtx3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\rnf180

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\trim69.2

1 of 13

Xtro\xnf7

1 of 13

Danio rerio (Zebrafish) (37)

Drer\toporsa

9 of 14

Yes

Drer\toporsb

8 of 14

Yes

Drer\pex10

2 of 14

Drer\amfrb

1 of 14

Drer\btr07

1 of 14

Yes

Drer\btr12

1 of 14

Drer\btr25

1 of 14

Yes

Drer\btr26

1 of 14

Yes

Drer\btr30

1 of 14

Drer\chfr

1 of 14

Drer\dtx3l2.2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\rnf185

1 of 14

Drer\rnf213b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:dkey-37m8.11

1 of 14

Yes

Drer\si:dkey-183n20.15

1 of 14

Yes

Drer\trim35-12

1 of 14

Yes

Drer\trim35-24

1 of 14

Yes

Drer\trim107

1 of 14

Yes

Drer\trim109

1 of 14

Yes

Drer\zgc:173581

1 of 14

Yes

Drer\zmp:0000000662

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\C32D5.11

3 of 14

Yes

Cele\C55A6.1

1 of 14

Cele\F10D7.5

1 of 14

Cele\F54B11.5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\Y47G6A.31

1 of 14

Cele\Y105E8A.14

1 of 14

Cele\ZC13.1

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP005831

9 of 12

Yes

Agam\AgaP_AGAP000736

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (66)

Atha\AT2G39100

4 of 13

Yes

Atha\AT3G05250

4 of 13

Yes

Atha\AT3G05670

2 of 13

Yes

Atha\PEX10

2 of 13

Atha\AIP2

1 of 13

Atha\AT1G12760

1 of 13

Yes

Atha\AT1G18660

1 of 13

Atha\AT1G19310

1 of 13

Atha\AT1G22510

1 of 13

Yes

Atha\AT1G24440

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT1G68070

1 of 13

Yes

Atha\AT1G72175

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT2G42030

1 of 13

Atha\AT2G44410

1 of 13

Atha\AT2G45135

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\IRC20

1 of 13

Yes

Scer\PEX10

1 of 13

Yes

Scer\PSH1

1 of 13

Yes

Scer\RAD18

1 of 13

Yes

Scer\TUL1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (7)

Spom\brl2

1 of 12

Yes

Spom\hrd1

1 of 12

Yes

Spom\pas4

1 of 12

Yes

Spom\slx8

1 of 12

Yes

Spom\SPBC14F5.10c

1 of 12

Yes

Spom\SPBC16G5.03

1 of 12

Yes

Spom\SPCC548.05c

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Topors. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

CG18605

4 of 13

Pex10

2 of 13

Cbl

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TOPORS; TOP1 binding arginine/serine rich protein, E3 ubiquitin ligase

model of retinitis pigmentosa 31

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TOPORS; TOP1 binding arginine/serine rich protein, E3 ubiquitin ligase

10 of 14

609507

RETINITIS PIGMENTOSA 31; RP31

retinitis pigmentosa 31

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Topors - Cp190

anti bait coimmunoprecipitation, western blot

(Capelson and Corces, 2005)

Topors - Lam

two hybrid, anti bait coimmunoprecipitation, western blot

(Capelson and Corces, 2005)

Topors - Top1

pull down, autoradiography

(Secombe and Parkhurst, 2004)

Topors - hry

electrophoretic mobility shift assay, autoradiography, pull down, anti tag western blot, anti tag coimmunoprecipitation, enzymatic study, two hybrid

(Secombe and Parkhurst, 2004)

Topors - mod(mdg4)

anti bait coimmunoprecipitation, western blot, two hybrid, pull down, anti tag western blot

(Capelson and Corces, 2005)

Topors - p53

pull down, autoradiography

(Secombe and Parkhurst, 2004)

Topors - su(Hw)

two hybrid, anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Capelson and Corces, 2005)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cindr

enhanceable

Topors

(Ketosugbo et al., 2017)

suppressible

Topors

(Matsui et al., 2011)

hry

suppressible

Topors

(Secombe and Parkhurst, 2004)

suppressible

Topors

(Capelson and Corces, 2005)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9V8P9 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267351

MIST (protein-protein) - An integrated Molecular Interaction Database

37188

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-87

Cytogenetic map

56A2-56A2

Sequence location

2R:18,972,612..18,977,262 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56A2-56A2

Limits computationally determined from genome sequence between P{lacW}prod^k08810 and P{PZ}ena⁰²⁰²⁹&P{lacW}cora^k08713

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-87

(FlyBase, 2016)

2-92.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31091

y¹ v¹; P{TRiP.JF01556}attP2

18839

w¹¹¹⁸; PBac{WH}Topors^f05115/CyO

606951

cn¹ Topors^Z1837 bw¹/CyO

606952

cn¹ Topors^Z4522 bw¹/CyO

78925

y¹ w^*; TI{CRIMIC.TG4.1}Topors^{CR00491-TG4.1}

76975

y¹ sc^* v¹ sev²¹; P{TKO.GS00722}attP40

79441

y¹ sc^* v¹ sev²¹; P{TOE.GS01378}attP40

607291

y¹ w¹/Dp(1;Y)y⁺; Topors^fsF114SX19/CyO

34671

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01149}attP2

15104R-2

v105444

v10642

w¹¹¹⁸; P{GD4575}v10642

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Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (34)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD43109

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0267351

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Capelson and Corces, 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Topors is required for gypsy\su(Hw)BR insulator function.

(Capelson and Corces, 2005)

Identification: as a protein that interacts with h protein.

(Secombe and Parkhurst, 2004)

Topors protein mediates h protein polyubiquitination and can lead to h protein degradation.

(Secombe and Parkhurst, 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: Topors nbl

(Matsui et al., 2011)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Topors CG15104

(Secombe and Parkhurst, 2004)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Name Synonyms

Topoisomerase I-interacting protein

(Secombe and Parkhurst, 2004)

nuclear blebber

(Matsui et al., 2011, Matsui et al., 2007, Wakimoto et al., 2004)

Secondary FlyBase IDs

FBgn0034410
FBgn0069095

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37188

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V8P9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V8P9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KEZ9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V8P9

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37188/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267351

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267351

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15104

MARRVEL_MODEL - MARRVEL (model organism gene)

37188

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0267351

DroID - A comprehensive database of gene and protein interactions.

FBgn0267351

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0034410

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267351

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15104

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030677

MIST (protein-protein) - An integrated Molecular Interaction Database

37188

References (48)

The following external sites may use different assemblies or annotations than FlyBase.

2.24

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