FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Toporsf05115
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General Information
Symbol
Dmel\Toporsf05115
Species
D. melanogaster
Name
FlyBase ID
FBal0159657
Feature type
allele
Associated gene
Dmel\Topors
Associated Insertion(s)
PBac{WH}Toporsf05115
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Matsui et al., 2011)

amorphic allele - molecular evidence

(Matsui et al., 2011)
Mutagen
piggyBac activity
(Bloomington Drosophila Stock Center, 2004.11.6)
Progenitor genotype
Associated Insertion(s)
PBac{WH}Toporsf05115
Cytology
Description

Conceptual translation predicts that Toporsf05115 encodes a protein truncated after amino acid 47.

(Matsui et al., 2011)
Allele components
Component
Use(s)
Inserted element
PBac{WH}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous and transheterozygous males, but not females, show near random segregation of all chromosomes at meiosis I, but not meiosis II, as a result of non-disjunction. Male fecundity is also greatly reduced.

      Toporsf05115 spermatocytes exhibit abnormal separation of centrioles at meiosis I leading to tetrapolar spindles, and inappropriate/unresolved connections ('bridges') between homologs at anaphase I. At late prophase, mutant chromosomes appear slightly less condensed than normal. A greater proportion of cells are observed in prometaphase and at anaphase in the mutants compared to controls, suggesting a delay in the chromosome cycle.

      Nuclear blebbing is observed in late-stage Toporsf05115 spermatocytes, though it is variable. Blebbing is not observed in testis sheath cells, larval neuroblasts or the giant nuclei of the larval salivary gland cells.

      (Matsui et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      Toporsf05115 has abnormal meiotic cell cycle | male limited phenotype, non-enhanceable by mod(mdg4)Z3-5578/mod(mdg4)[+]

      (Matsui et al., 2011)

      Toporsf05115 has male semi-sterile phenotype, non-enhanceable by mod(mdg4)Z3-5578/mod(mdg4)[+]

      (Matsui et al., 2011)

      Toporsf05115 has abnormal meiotic cell cycle | male limited phenotype, non-enhanceable by mod(mdg4)Z3-5578

      (Matsui et al., 2011)

      Toporsf05115 has male semi-sterile phenotype, non-enhanceable by mod(mdg4)Z3-5578

      (Matsui et al., 2011)
      Enhancer of
      Statement
      Reference

      Toporsf05115 is an enhancer of visible phenotype of Scer\GAL4GMR.PF, cindrRNAi.PC.PD.UAS

      (Ketosugbo et al., 2017)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Toporsf05115 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, cindrRNAi.PC.PD.UAS

      (Ketosugbo et al., 2017)
      Suppressor of
      Statement
      Reference

      Topors[+]/Toporsf05115 is a suppressor of wing margin phenotype of ct6

      (Matsui et al., 2011)
      NOT Suppressor of
      Statement
      Reference

      Toporsf05115/Toporsf05115 is a non-suppressor of wing margin phenotype of ct6

      (Matsui et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In Toporsf05115/+; mod(mdg4)Z3-5578 males, individual lagging chromosome are observed but no anaphase I bridges are seen. In both Toporsf05115; mod(mdg4)Z3-5578/+ and Toporsf05115; mod(mdg4)Z3-5578 males, nearly all anaphase/telophase figures have a bridge.

      The near sterility and nondisjunction phenotypes associated with Toporsf05115 are not improved in a mod(mdg4)Z3-5578 hetero- or homozygous background.

      mod(mdg4)Z3-5578, Toporsf05115 double mutants show a meiotic chromsome condensation defect that is more severe than either single mutant.

      Heterozygosity, but not homozygosity, for Toporsf05115 restores the wing margin of ct6 flies.

      (Matsui et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Toporsf05115
      (Binder et al., 2025)
      dtoporsf05115
      (Matsui et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)