FlyBase Allele Report: Dmel\mod(mdg4)[Z3-5578]

General Information

Symbol

Dmel\mod(mdg4)^Z3-5578

Species

D. melanogaster

Name

FlyBase ID

FBal0194707

Feature type

allele

Associated gene

Dmel\mod(mdg4)

Associated Insertion(s)

Carried in Construct

Also Known As

mnm^Z3-5578

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Thomas et al., 2005)

Mutagen

ethyl methanesulfonate

(Thomas et al., 2005)

Progenitor genotype

Cytology

Description

The stop mutation is in the C-terminal coding exon, which is present in most mod(mdg4) isoforms.

(Thomas et al., 2005)

Amino acid replacement: W449term.

(Thomas et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Matsui et al., 2011, Thomas et al., 2005)

abnormal meiotic cell cycle | male

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with Df(3R)GC14)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ10)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^142Δ33)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^B2)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^eGp4)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^T16)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | male (with mod(mdg4)^Z3-3298)

(Soltani-Bejnood et al., 2007)

abnormal meiotic cell cycle | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

female fertile (with Df(3R)GC14)

(Soltani-Bejnood et al., 2007)

viable

(Soltani-Bejnood et al., 2007)

Phenotype Manifest In

autosome | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

G2 phase & nuclear chromosome

(Thomas et al., 2005)

meiosis I & nuclear chromosome

(Thomas et al., 2005)

nucleus | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

sex chromosome | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

spermatocyte | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

spermatocyte cyst | spermatogenesis (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

testis | adult stage (with mod(mdg4)^Z3-3298)

(Sun et al., 2019)

Detailed Description

Statement

Reference

mod(mdg4)^Z3-3298/mod(mdg4)^Z3-5578 males exhibit defects in chromosome segregation (i.e. random segregation of sex chromosomes) and conjunction during meiosis I; abnormal chromosome territories during S5, S6 and prometaphase I stages; an increased number of smaller DNA blobs (chromosome masses) during prometaphase I; prematurely separated bivalents into univalents during meiosis I; and a striking size variation among the nuclei present in spermatid cysts compared to controls.

(Sun et al., 2019)

mod(mdg4)^Z3-5578 spermatocytes show precocious separation of homologs and reduced chromosome condensation.

(Matsui et al., 2011)

Homozygous males show a high frequency of X-Y chromosome nondisjunction in meiosis (51.2%).

mod(mdg4)^Z3-5578/Df(3R)GC14 males show a high frequency of 4th chromosome loss (28.1%) and chromosome 2 nondisjunction (43.3%) in meiosis.

mod(mdg4)^Z3-5578/Df(3R)GC14 females do not show increased X chromosome nondisjunction compared to controls and the frequency of recombination in large intervals on the X and third chromosomes in the mutant females is normal.

(Soltani-Bejnood et al., 2007)

mod(mdg4)^Z3-5578 mutants show elevated frequencies of autosomal and sex chromosome nondisjunction. Analysis of mutant spermatocytes shows that nondisjunction is limited largely to meiosis I. Premeiotic and meiotic homolog pairing are normal. The earliest observable phenotype is that of "territory expansion" in late G2, with homologous chromosomes being at a greater distance from one another than in wild-type spermatocytes. mod(mdg4)^Z3-5578 spermatocytes show incomplete chromatin condensation and form up to eight large clumps of chromatin form by metaphase I, instead of the one central mass observed in wild type. The clumps represent bivalents and univalents, consistent with a defect in formation or maintenance of bivalents. In early anaphase I, chromosomes are distributed unevenly and asymmetrically along the spindle. In late anaphase I, there is a high frequency of laggards and polar chromatin masses of unequal size, indicating high frequency of nondisjunction.

(Thomas et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

mod(mdg4)^Z3-5578/mod(mdg4)[+] is a non-enhancer of abnormal meiotic cell cycle | male limited phenotype of Topors^f05115

(Matsui et al., 2011)

mod(mdg4)^Z3-5578/mod(mdg4)[+] is a non-enhancer of male semi-sterile phenotype of Topors^f05115

(Matsui et al., 2011)

mod(mdg4)^Z3-5578 is a non-enhancer of abnormal meiotic cell cycle | male limited phenotype of Topors^f05115

(Matsui et al., 2011)

mod(mdg4)^Z3-5578 is a non-enhancer of male semi-sterile phenotype of Topors^f05115

(Matsui et al., 2011)

mod(mdg4)^Z3-5578/mod(mdg4)^T16 is a non-enhancer of abnormal body color phenotype of y²

(Soltani-Bejnood et al., 2007)

NOT Suppressor of

Statement

Reference

mod(mdg4)^Z3-5578/mod(mdg4)^T16 is a non-suppressor of abnormal body color phenotype of y²

(Soltani-Bejnood et al., 2007)

Other

Statement

Reference

Topors^f05115, mod(mdg4)^Z3-5578 has abnormal meiotic cell cycle phenotype

(Matsui et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

In Topors^f05115/+; mod(mdg4)^Z3-5578 males, individual lagging chromosome are observed but no anaphase I bridges are seen. In both Topors^f05115; mod(mdg4)^Z3-5578/+ and Topors^f05115; mod(mdg4)^Z3-5578 males, nearly all anaphase/telophase figures have a bridge.

The near sterility and nondisjunction phenotypes associated with Topors^f05115 are not improved in a mod(mdg4)^Z3-5578 hetero- or homozygous background.

mod(mdg4)^Z3-5578, Topors^f05115 double mutants show a meiotic chromsome condensation defect that is more severe than either single mutant.

(Matsui et al., 2011)

Body colour: the y² phenotype (black bristles, yellow body and wings) is not altered by mod(mdg4)^Z3-5578/mod(mdg4)^T16.

(Soltani-Bejnood et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

mod(mdg4)^142Δ49

(Soltani-Bejnood et al., 2007)

mod(mdg4)^T6

(Soltani-Bejnood et al., 2007)

Partially complements

mod(mdg4)^Z3-3401

(Soltani-Bejnood et al., 2007)

mod(mdg4)^eGp4

(Soltani-Bejnood et al., 2007)

mod(mdg4)^142Δ33

(Soltani-Bejnood et al., 2007)

mod(mdg4)^142Δ10

(Soltani-Bejnood et al., 2007)

mod(mdg4)^T16

(Soltani-Bejnood et al., 2007)

mod(mdg4)^B2

(Soltani-Bejnood et al., 2007)

Fails to complement

mod(mdg4)^Z3-3298

(Soltani-Bejnood et al., 2007)

Rescued by

mod(mdg4)^Z3-5578/mod(mdg4)^Z3-3298 is rescued by Scer\GAL4^VP16.bam/mod(mdg4)^{UAS.mnm.C.EGFP}

(Sun et al., 2019)

mod(mdg4)^Z3-5578/mod(mdg4)^Z3-3298 is rescued by mod(mdg4)^UAS.mnm/Scer\GAL4^VP16.bam

(Sun et al., 2019)

mod(mdg4)^Z3-5578 is rescued by mod(mdg4)^MNM.hs.EGFP

(Thomas et al., 2005)

Partially rescued by

mod(mdg4)^Z3-5578/mod(mdg4)^Z3-3298 is partially rescued by mod(mdg4)^{βTub85D.mnm.C.EGFP}

(Sun et al., 2019)

mod(mdg4)^Z3-5578/mod(mdg4)^Z3-3298 is partially rescued by mod(mdg4)^{UAS.mnm.N.EGFP}/Scer\GAL4^VP16.bam

(Sun et al., 2019)

Not rescued by

mod(mdg4)^Z3-5578/mod(mdg4)^Z3-3298 is not rescued by Scer\GAL4^VP16.bam/Zzzz\VHH^{deGradFP.βTub85D}/mod(mdg4)^{UAS.mnm.C.EGFP}

(Sun et al., 2019)

Comments

mod(mdg4)^Z3-5578 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, but they fail to complement mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing a high level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^T16 (44.3%), mod(mdg4)^142Δ10 (46%), mod(mdg4)^142Δ33 (37.3%), mod(mdg4)^eGp4 (50.3%) and mod(mdg4)^B2 (37.3%).

mod(mdg4)^Z3-5578 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, and they partially or fully complement mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing only a low level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^142Δ15 (0.56%), mod(mdg4)^142Δ32 (0.95%), mod(mdg4)^142Δ29 (1.54%), mod(mdg4)^142Δ49 (0%), mod(mdg4)^neo129 (4.46%), mod(mdg4)⁰² (1.72%), mod(mdg4)⁰³ (0.07%), mod(mdg4)²⁰ (2.96%), mod(mdg4)¹¹⁷ (0.75%), mod(mdg4)²⁶⁹ (1.44%), mod(mdg4)³²⁴ (2.84%) and mod(mdg4)³⁴⁰ (1.89%).

mod(mdg4)^Z3-3401 strongly (but not completely) complements mod(mdg4)^Z3-5578; transheterozygotes show only a low level (1.42%) of X-Y nondisjunction in male meiosis.

mod(mdg4)^T6 fully complements mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis.

mod(mdg4)^Z3-5578 fails to complement mod(mdg4)^Z3-3298 with respect to X-Y chromosome nondisjunction in male meiosis; transheterozygotes show 45.2% X-Y nondisjunction.

(Soltani-Bejnood et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

606953

bw¹; st¹ mod(mdg4)^Z3-5578/TM3, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Z3-5578

(Soltani-Bejnood et al., 2007)

mnm^Z3-5578

(Weber et al., 2020, Sun et al., 2019, Blattner et al., 2016, Thomas and McKee, 2007, Thomas et al., 2005)

mnm^Z5578

(Matsui et al., 2011)

mnm^z3-5578

(Kabakci et al., 2022, Vernizzi and Lehner, 2021)

mod(mdg4)^Z3-5578

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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