Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

A heat-stable variant of His1 was used to permit experiments at 35oC.

Interaction in vitro; linker histone produced as a recombinant fusion protein in bacterial system; core histones derived from wild-type flies.

Nucleosome arrays were mixed with linker histone His1 at various concentrations to determine the effect on chromatin structure, as assessed by analytical ultracentrifugation.