FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000100832
Interaction Network
Interactions Browser links
pum network
mei-P26 network
Reported Interactions
FBrf0223419-5.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

mei-P26

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Experiments were done in bam[Δ86] mutant ovaries, which contain only undifferentiated pre-cystoblasts.

FBrf0223419-8.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mei-P26

bait

biotin tag

pum 

Pum

prey

[35]S label

pum 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pum 
C-terminal region
sufficient binding region
aa 849–1533
three-prime UTR
sufficient binding region
nt 844-1207

fragment 2

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced and labeled by in vitro translation.

Positive control.

FBrf0240993-6.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pum 

Pum

bait

mei-P26

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)