FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Atg9, Traf6
FlyBase ID
FBig0000100880
Interaction Network
Interactions Browser links
Atg9 network
Traf6 network
Reported Interactions
FBrf0223739-1.coIP.WB
Reference
Tang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg9 

Atg9

bait
Traf6 

dTRAF2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult gut of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Tang et al., 2013)
FBrf0223739-2.coIP.WB
Reference
Tang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Traf6 

dTRAF2

bait

FLAG tag

Atg9 

Atg9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Tang et al., 2013)
FBrf0223739-3.PD.WB
Reference
Tang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg9 

Atg9

bait

GST tag

Atg9 
Traf6 

dTRAF2

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atg9 
C-terminal region
sufficient binding region
aa 668-845
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Tang et al., 2013)
FBrf0223739-4.coIP.WB
Reference
Tang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult gut / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg9 

Atg9

bait
Traf6 

dTRAF2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult gut of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Tang et al., 2013)

The Atg9-Traf6 interaction is abrogated by rapamycin-induced autophagy.

(Tang et al., 2013)

Knockdown of Atg12 leads to a sustained Atg9-Traf6 interaction upon Erwinia infection.

(Tang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)