Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Utx binds EcR/usp in both the presence and absence of ecdysone.

GST-Utx was mixed with both EcR and usp simultaneously for the pull down.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Utx binds EcR in both the presence and absence of ecdysone.