FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Gαo, kermit
FlyBase ID
FBig0000100947
Interaction Network
Interactions Browser links
Gαo network
kermit network
Reported Interactions
FBrf0223257-1.PD.WB
Reference
Lin and Katanaev, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαo 

Gαo

bait

His tag

kermit 

Kermit

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

kermit bound Gαo in the presence of both GDP and GTP.

(Lin and Katanaev, 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lin and Katanaev, 2013)
FBrf0223257-2.PD.WB
Reference
Lin and Katanaev, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kermit 

Kermit

bait

MBP tag

Gαo 

Gαo

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Lin and Katanaev, 2013)
FBrf0223257-3.PD.WB
Reference
Lin and Katanaev, 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kermit 

Kermit

bait

MBP tag

Gαo 

Gαo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult head extract.

(Lin and Katanaev, 2013)

kermit binds Gαo but not Dhit.

(Lin and Katanaev, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)