FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lgs, pygo
FlyBase ID
FBig0000100998
Interaction Network
Interactions Browser links
lgs network
pygo network
Reported Interactions
FBrf0146918-4.Y2H
Reference
Kramps et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lgs 

Lgs

bait

fused to LexA DNA-binding domain

lgs 
pygo 

Pygo

prey

fused to B42 activation domain

pygo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lgs 
N-terminal region
sufficient binding region
aa 1-732

coordinates relative to lgs-PA

pygo 
PHD domain
sufficient binding region
aa 742-815

coordinates relative to pygo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Three different pygo clones identified in two independent lgs two hybrid screens.

(Kramps et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Kramps et al., 2002)

Two-hybrid system: yeast LexA-BD/B42-AD

(Kramps et al., 2002)
FBrf0146918-5.Y2H
Reference
Kramps et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lgs 

Lgs

bait

fused to LexA DNA-binding domain

lgs 
lgs 
pygo 

Pygo

prey

fused to B42 activation domain

pygo 
pygo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lgs 
HD1 region
sufficient binding region
aa 231-430

coordinates relative to lgs-PA

lgs 
HD1 region
necessary binding region
aa 318-345

coordinates relative to lgs-PA

pygo 
PHD domain
sufficient binding region
aa 735-815

coordinates relative to pygo-PA

pygo 
PHD domain
necessary binding region
aa 735-815

coordinates relative to pygo-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Kramps et al., 2002)

Two-hybrid system: yeast LexA-BD/B42-AD

(Kramps et al., 2002)
FBrf0174483-1.coIP.WB
Reference
Thompson, 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

Pygo

bait

HA tag

lgs 

Lgs

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Thompson, 2004)
FBrf0174791-1.PD.A
Reference
Townsley et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

Pygo

bait

GST tag

pygo 
pygo 
pygo 
pygo 
pygo 
pygo 
lgs 

Lgs

prey

[35]S label

lgs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
PHD domain
sufficient binding region
aa 747-815

coordinates relative to pygo-PA

pygo 
PHD domain residue C753
mutation disrupting interaction
aa 753

C753A, coordinates relative to pygo-PA

pygo 
PHD domain residue L781
mutation disrupting interaction
aa 781

L781A, coordinates relative to pygo-PA

pygo 
PHD domain residue T782
mutation disrupting interaction
aa 782

T782A, coordinates relative to pygo-PA

pygo 
PHD domain residue L789
mutation disrupting interaction
aa 789

L789A, coordinates relative to pygo-PA

pygo 
PHD domain residue A785
mutation disrupting interaction
aa 785

A785V, coordinates relative to pygo-PA

lgs 
HD1 and HD2 regions
sufficient binding region
aa 233-555

coordinates relative to lgs-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Townsley et al., 2004)
FBrf0187313-1.Y2H
Reference
Hoffmans et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lgs 

Lgs

bait

fused to LexA DNA-binding domain

pygo 

Pygo

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hoffmans et al., 2005)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Hoffmans et al., 2005)
FBrf0208546-3.coIP.WB
Reference
Kessler et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pygo 

dPygo

bait

V5 tag

pygo 
pygo 
lgs 

Lgs

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
PHD domain residue 757
mutation decreasing interaction
aa 757

L757E

pygo 
PHD domain residue 789
mutation disrupting interaction
aa 789

L789A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kessler et al., 2009)
FBrf0208546-4.coIP.WB
Reference
Kessler et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
lgs 

Lgs

bait

HA tag

pygo 

dPygo

prey

V5 tag

pygo 
pygo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
PHD domain residue 757
mutation decreasing interaction
aa 757

L757E

pygo 
PHD domain residue 789
mutation disrupting interaction
aa 789

L789A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kessler et al., 2009)
FBrf0208546-5.PD.A
Reference
Kessler et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

dPygo

bait

GST tag

pygo 
pygo 
pygo 
lgs 

Lgs

prey

[35]S label

lgs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
PHD domain
sufficient binding region
aa 739-815
pygo 
PHD domain residue 757
mutation disrupting interaction
aa 757

L757E

pygo 
PHD domain residue 789
mutation disrupting interaction
aa 789

L789A

lgs 
interaction domain
sufficient binding region
aa 231-555
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kessler et al., 2009)
FBrf0223501-1.XC
Reference
Miller et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pygo 

Pygo

neutral component
pygo 

Pygo

neutral component
pygo 
pygo 

Pygo

neutral component
pygo 
lgs 

Lgs

neutral component
lgs 
lgs 

Lgs

neutral component
lgs 

Lgs

neutral component
lgs 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pygo 
PHD domain
sufficient binding region
aa 747-808
lgs 
HD1 domain
sufficient binding region
aa 321-355
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Miller et al., 2013)

The single α helix (α1) and adjacent β strand (&bgr5) of pygo contact the lgs HD1 domain.

(Miller et al., 2013)

Unlike its human ortholog, fly pygo PHD domain has an altered His3 binding pocket with an apparent groove for binding of the His3-Arg2 residue.

(Miller et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)