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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000101033
Interaction Network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0205145-1.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

Rpd3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was salivary glands of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

FBrf0223699-11.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Rpd3

bait

FLAG tag

Gug 

Atro

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The Gug-Rpd3 complex associates with a ci binding site at the dpp locus (ChIP experiment).

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0223699-12.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

FLAG tag

Gug 

Rpd3

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
N-terminal region
sufficient binding region

\'AtroN\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The Gug-Rpd3 complex associates with a ci binding site at the dpp locus (ChIP experiment).

FBrf0223699-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gug 

Atro

bait

Myc tag

Rpd3

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Tagged Rpd3-T7, Gug-Myc and ci-FLAG were co-transfected and subjected to sequential FLAG then Myc immunoprecipitation to test for ternary complexes.

Based on two-step coimmunoprecipitation, ci, Gug and Rpd3 exist in a larger complex togther.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)