Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

drl and Src64B specifically co-immunoprecipitate in the presence or absence of Wnt5 protein. Src64B tyrosine kinase activity is required for the formation and stability of the drl-Src64B complex.

Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from transfected construct.

drl and Src64B specifically co-immunoprecipitate in the presence or absence of Wnt5 protein.

Source was mammalian cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Interaction of Src64B with drl\'s intracellular domain requires the Src64B SH2 domain and kinase activity, but not the SH3 domain.

Source was mouse SYF cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction of Src64B with the drl intracellular domain requires the Src64B SH2 domain and kinase activity, but not the SH3 domain.

Two-hybrid system: mammalian GAL4-BD/VP16-AD.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

A mutation that disrupts drl homodimerization decreases the interaction of drl with Src64B.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Kinase dead Src64B does not compete with wild-type Src64B for binding to drl.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Forced dimerization of drl, by replacement of the drl extracellular domain by the IgG Fc domain, increases the interaction with Src64B.