Bait derived from in vitro transcription.

Prey endogenous to embryonic extract.

Streptavidin pull down of nos +2\'-3X RNA element via S1 aptamer in TRAP tag.

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant protein in bacterial system.

Mutation of the CGUU motifs in the nos +2\' RNA element disrupts interaction with rump protein in vitro, and disrupts posterior oocyte localization of transgenic transcripts.

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type ovarian extract.

The 75 kDa binding activity to the nos +2\' element is absent in rump¹ mutant ovaries.

The interaction of rump protein and nos RNA is not observed in rump¹ mutant ovaries.

Bait endogenous to dissected ovary extract.

Prey endogenous to dissected ovary extract.

rump protein immunoprecipitated from dissected ovary extract and the presence of coimmunoprecipitated nos RNA detected by RT-PCR.

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

Positive control.