FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Vang, pk
FlyBase ID
FBig0000101472
Interaction Network
Interactions Browser links
Vang network
pk network
Reported Interactions
FBrf0160398-2.coIP.WB
Reference
Bastock et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Stbm

bait

FLAG tag

pk 

Pk

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bastock et al., 2003)
FBrf0160398-3.coIP.WB
Reference
Bastock et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

Myc tag

Vang 

Stbm

prey

CD2 fusion

Vang 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
intracellular domain
sufficient binding region
aa 298-578

lacks final three residues of PDZ binding motif

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bastock et al., 2003)

Vang intracellular domain fused to rat CD2 membrane protein.

(Bastock et al., 2003)
FBrf0162094-1a.Y2H
Reference
Jenny et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Stbm

bait
pk 

Pk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Jenny et al., 2003)

Two-hybrid system: yeast

(Jenny et al., 2003)
FBrf0162094-2.PD.A
Reference
Jenny et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GST tag

pk 
Vang 

Stbm

prey

[35]S label

Vang 
Vang 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
intracellular domain
sufficient binding region
aa 297-444

"ΔC1" construct

Vang 
intracellular domain
necessary binding region
aa 361-444

"ΔC2" construct

pk 
C-terminal region
sufficient binding region
aa 778-963

coordinates relative to pk-PA, construct "ΔN1"

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jenny et al., 2003)
FBrf0213849-3.PD.A
Reference
Mirkovic et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GST tag

pk 
Vang 

Stbm

prey

[35]S label

Vang 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
PkC
sufficient binding region

This assay uses "the part of Pk common to all isoforms" but makes no further specifications. Further investigation into the citations also yields no specification beyond that these constructs encapsulate binding domains common to all isoforms of pk that are found in the last two exons of the gene.

Vang 
C-terminal Tail
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mirkovic et al., 2011)
FBrf0222158-1.coIP.WB
Reference
Strutt et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pk 

Pk

bait

GFP tag

Vang 

Stbm

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The C-terminal CAAX farnesylation motif of Pk is dispensable for binding to Vang in this assay.

(Strutt et al., 2013)

Source was extract of cell line; bait produced from transfected construct; prey produced from transfected construct.

(Strutt et al., 2013)
FBrf0225692-3.coIP.WB
Reference
Ayukawa et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Stbm

bait

FLAG tag

pk 

Pk

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ayukawa et al., 2014)
FBrf0245453-3.coIP.WB
Reference
Humphries et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

FLAG tag

Vang 
pk 

Pk

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
interaction domain
mutation decreasing interaction
aa 317aa 318aa 574

D314E, coordinates relative to Vang-PA

R318L, coordinates relative to Vang-PA

K574H, coordinates relative to Vang-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Humphries et al., 2020)
FBrf0257118-1.coIP.WB
Reference
Humphries et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

FLAG tag

Vang 
pk 

Pk

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal domain residues 374
mutation disrupting interaction
aa 374

Y374A, Y374F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Humphries et al., 2023)

Vang interaction pk requires aa 374 to be charged.

(Humphries et al., 2023)
FBrf0257118-5.PD.WB
Reference
Humphries et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

Bead conjugated

Vang 
pk 

Pk

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal domain
sufficient binding region
aa 367-381

within the context of aa 367-381

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide chemically synthesized.; prey derived from transfected construct.

(Humphries et al., 2023)

Phosphorylated aa 374 Vang interacts more strongly to pk than dsh.

(Humphries et al., 2023)
FBrf0257118-6.PD.WB
Reference
Humphries et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

Bead conjugated

Vang 
pk 

Pk

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Vang 
C terminal phosphorylation domain residue 374
mutation increasing interaction
aa 374

within the context of aa 367-381

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

pk reduces interaction with phosphorylated Vang peptide and dsh, but not non-phosphorylated Vang and dsh.

(Humphries et al., 2023)

Interaction in vitro; bait peptide chemically synthesized.; prey derived from transfected construct.

(Humphries et al., 2023)
FBrf0258261-2.PL.WB
Reference
Song et al., 2023
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

APEX tag

pk 

Pk

prey

Biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Song et al., 2023)
FBrf0258261-4.PL.MS
Reference
Song et al., 2023
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vang 

Vang

bait

APEX tag

pk 

Pk

prey

Biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Song et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)