Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Using run bait for this two hybrid screen, 10 of 90 independent clones represented Bro.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Bro strongly enhances the binding of run to [32]P-labeled DNA comprising the polyomavirus enhancer, which contains a PEBP2/CBF binding site. Alone, run has only weak DNA binding activity, and Bgb has none at all.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

HGScore = 15.053051