Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The brat coiled coil domain is necessary for binding to mira aa 369-508, but not to mira aa 527-638.

The brat NHL domain is likely to interact with mira aa 527-638.

brat B boxes are dispensable for binding to mira

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

The addition of recombinant stau did not affect the mira-brat interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Kd = 10.4uM