Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Copurified proteins were identified only in sqd immunoprecipitation, and not in the negative control (dl immunoprecipitation).

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

cup interacts with both HA-tagged sqd isoforms tested (SqdA and SqdS).

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The sqd-cup interaction is RNA-dependent (disrupted by RNase A).