Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo or ovary cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Affinity purification of bait and identification of prey by MALDI peptide mapping.
Source was soluble cytosolic extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
