Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult testis extract.
Source was testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast lexA-BD/B42-AD
