Source was live S2 cells; bait produced from transfected construct; prey produced from transfected construct.

Orc6 forms dimers in vivo at the plasma membrane, but not in the nucleus or cytoplasm, as deduced by FRET analysis of live S2 cells.

Source was cell extract of baculovirus-infected cell line; protein produced and purified from transfected construct.

Orc6 forms dimers in vitro.

Orc6 dimerization is important for Orc6-facilitated septin filament formation.

Purified Orc6 was separated by size exclusion chromatography and fractions analyzed by western blot.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Orc6 forms dimers in vitro.

Orc6 dimerization is important for Orc6-facilitated septin filament formation.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Orc6 forms dimers in vitro.

Orc6 dimerization is important for Orc6-facilitated septin filament formation.