FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Usp2, Rpt6
FlyBase ID
FBig0000102284
Interaction Network
Interactions Browser links
Usp2 network
Rpt6 network
Reported Interactions
FBrf0225968-4.coIP.WB
Reference
Engel et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Usp2 

USP2

bait

Myc tag

Rpt6 

Pros45

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Engel et al., 2014)

The dependency of the Usp2-Rpt6 interaction on co-transfection of imd is consistent with the model that Usp2 recruits the proteasome to imd.

(Engel et al., 2014)
FBrf0225968-5.PD.MS
Reference
Engel et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Usp2 

USP2

bait

GST tag

Rpt6 

Pros45

prey

7 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Engel et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)